Enter the dilution factor of the sample and the light absorbance at 430 nanometers into the Calculator. The calculator will evaluate the EBC. 

EBC Formula

EBC = 25 * D * A430

Variables:

  • EBC is the EBC ()
  • D is the dilution factor of the sample
  • A430 is the light absorbance at 430 nanometers

To calculate EBC, multiply the dilution factor by 25, then multiply by the light absorbance at a 430 nanometers wavelength.

How to Calculate EBC?

The following steps outline how to calculate the EBC.

  1. First, determine the dilution factor of the sample. 
  2. Next, determine the light absorbance at 430 nanometers. 
  3. Next, gather the formula from above = EBC = 25 * D * A430.
  4. Finally, calculate the EBC.
  5. After inserting the variables and calculating the result, check your answer with the calculator above.

Example Problem : 

Use the following variables as an example problem to test your knowledge.

dilution factor of the sample = 30

light absorbance at 430 nanometers = 12