Enter the slope into the calculator to determine the qpcr Efficiency. 

qpcr Efficiency Formula

The following formula is used to calculate the qpcr Efficiency. 

E = -1 + 10 ^ {-1/S}

E = -1 + 10 ^ (-1/S)

  • Where E is the qpcr Efficiency (%)
  • S is the slope 

To calculate the QPCR efficiency, raise 10 to the power of the negative inverse of the slope, then subtract 1 from the result.

How to Calculate qpcr Efficiency?

The following two example problems outline how to calculate the qpcr Efficiency.

Example Problem #1:

  1. First, determine the slope. In this example, the slope is given as 1.5.
  2. Finally, calculate the qpcr Efficiency using the equation above: 

E = -1 + 10 ^ (-1/S)

Inserting the values from above and solving the equation: 

E = -1 + 10 ^ (-1/1.5) = .784 (%)

Example Problem #2: 

Using the same process as above, first define the variables required by the formula. In this case, these values are:

slope = 4.5

Entering these values yields:

E = -1 + 10 ^ (-1/4.5) =-.40 (%)