Enter the absorbance of the test sample, the blank sample, and the 100% hemolysis sample into the calculator to determine the Hemolysis Index.

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## Hemolysis Index Formula

The following formula is used to calculate the Hemolysis Index.

HI = (A1 - A2) / (A3 - A2)

Variables:

- HI is the Hemolysis Index
- A1 is the absorbance of the test sample
- A2 is the absorbance of the blank sample
- A3 is the absorbance of the 100% hemolysis sample

To calculate the Hemolysis Index, subtract the absorbance of the blank sample from the absorbance of the test sample. Then subtract the absorbance of the blank sample from the absorbance of the 100% hemolysis sample. Divide the first result by the second result to get the Hemolysis Index.

## What is a Hemolysis Index?

A Hemolysis Index is a laboratory measurement used to determine the level of hemolysis, or the breakdown of red blood cells, in a blood sample. This index is important because high levels of hemolysis can interfere with certain blood tests and potentially lead to inaccurate results. The index is typically measured on a scale, with higher values indicating greater levels of hemolysis.

## How to Calculate Hemolysis Index?

The following steps outline how to calculate the Hemolysis Index (HI).

- First, determine the absorbance of the test sample (A1).
- Next, determine the absorbance of the blank sample (A2).
- Next, determine the absorbance of the 100% hemolysis sample (A3).
- Next, use the formula HI = (A1 – A2) / (A3 – A2) to calculate the Hemolysis Index.
- Finally, insert the values of A1, A2, and A3 into the formula and calculate the Hemolysis Index.

**Example Problem:**

Use the following variables as an example problem to test your knowledge.

A1 (absorbance of the test sample) = 0.8

A2 (absorbance of the blank sample) = 0.2

A3 (absorbance of the 100% hemolysis sample) = 1.0