Enter the concentration in µg/mL and the molecular weight in g/mol into the calculator to convert to µM (micromolar).

µg/mL to µM Conversion Formula

The following formula is used to convert µg/mL to µM.

µM = (µg/mL / MW) * 1000

Variables:

  • µM is the concentration in micromolar
  • µg/mL is the concentration in micrograms per milliliter
  • MW is the molecular weight in grams per mole (g/mol)

To convert µg/mL to µM, divide the concentration in µg/mL by the molecular weight in g/mol and then multiply by 1000 to convert to micromolar.

What is µg/mL to µM Conversion?

Converting µg/mL to µM is a common task in chemistry and biology, particularly in the context of preparing solutions for experiments. The concentration in µg/mL measures the mass of a substance in micrograms present in one milliliter of solution, while µM is a measure of the number of moles of a substance in one liter of solution. This conversion is necessary when the molecular weight of the substance is known and one needs to express concentration in terms of molarity, which is a more useful unit in chemical reactions.

How to Calculate µg/mL to µM?

The following steps outline how to convert µg/mL to µM.


  1. First, determine the concentration in µg/mL.
  2. Next, determine the molecular weight (MW) of the substance in g/mol.
  3. Use the formula µM = (µg/mL / MW) * 1000.
  4. Finally, calculate the concentration in µM.
  5. After inserting the variables and calculating the result, check your answer with the calculator above.

Example Problem : 

Use the following variables as an example problem to test your knowledge.

Concentration (µg/mL) = 150 µg/mL

Molecular Weight (g/mol) = 300 g/mol