Enter the volume and molarity of the Histidine and the weight of the Histidine into the calculator to determine the concentration of the Histidine buffer.

Histidine Buffer Formula

The following formula is used to calculate the concentration of a Histidine buffer. 

C = (V * M) / W

Variables:

  • C is the concentration of the Histidine buffer (M)
  • V is the volume of the solution (L)
  • M is the molarity of the Histidine (M)
  • W is the weight of the Histidine (g)

To calculate the concentration of a Histidine buffer, multiply the volume of the solution by the molarity of the Histidine. Then, divide the result by the weight of the Histidine.

What is a Histidine Buffer?

A histidine buffer is a type of buffering agent used in biological and chemical research to maintain a stable pH level in a solution. It is made from the amino acid histidine, which has the unique property of being able to donate or accept protons, allowing it to resist changes in pH. This makes it particularly useful in experiments involving proteins and other biological molecules, as it helps to preserve their structure and function by preventing pH fluctuations.

How to Calculate Histidine Buffer?

The following steps outline how to calculate the concentration of the Histidine buffer.

  1. First, determine the volume of the solution (V) in liters.
  2. Next, determine the molarity of the Histidine (M).
  3. Next, determine the weight of the Histidine (W) in grams.
  4. Next, gather the formula from above = C = (V * M) / W.
  5. Finally, calculate the concentration of the Histidine buffer (C).
  6. After inserting the variables and calculating the result, check your answer with the calculator above.

Example Problem : 

Use the following variables as an example problem to test your knowledge.

volume of the solution (V) = 0.5 L

molarity of the Histidine (M) = 0.1 M

weight of the Histidine (W) = 2 g