Convert nM to ng/mL for cortisol, estradiol, testosterone, thyroxine, vitamin D3, progesterone, insulin, or any custom molecular weight.

nM to ng/mL Converter

Convert nM to ng/mL using a selected analyte or a custom molecular weight.

Common analyte
Custom MW

nM to ng/mL Formula

The following formula is used to convert a concentration from nanomolar to nanograms per milliliter.

ng/mL = nM \times MW / 1000

Variables:

  • ng/mL is the mass concentration in nanograms per milliliter
  • nM is the molar concentration in nanomoles per liter
  • MW is the molecular weight of the compound in grams per mole

To calculate ng/mL, multiply the concentration in nM by the molecular weight of the compound and divide by 1000. If you need to reverse the calculation, use nM = ng/mL × 1000 ÷ MW.

What is nM to ng/mL Conversion?

A value in nM describes how many nanomoles of a substance are present per liter, while ng/mL describes the mass of that substance per milliliter. These units measure concentration in different ways, so the molecular weight of the compound is required to convert between them. Heavier molecules produce a larger ng/mL value at the same nM concentration.

This type of conversion is common in laboratory testing, pharmacology, endocrinology, and biomedical research because assay results may be reported in either molar units or mass-per-volume units depending on the method used.

How to Convert nM to ng/mL?

The following steps outline how to convert nM to ng/mL.


  1. First, determine the concentration in nanomolar (nM).
  2. Next, determine the molecular weight (MW) of the molecule in grams per mole.
  3. Multiply the nM value by the molecular weight.
  4. Divide the result by 1000 to convert the value to ng/mL.
  5. Check your answer using the calculator above.

Example Problem:

Use the following values as an example problem to test your knowledge.

Concentration = 10 nM

Molecule = Cortisol

Molecular Weight = 362.46 g/mol

ng/mL = 10 × 362.46 ÷ 1000 = 3.6246 ng/mL

Common Molecular Weights

Some approximate molecular weights often used in clinical and research settings are listed below.

  • Cortisol: 362.46 g/mol
  • Estradiol: 272.38 g/mol
  • Testosterone: 288.42 g/mol
  • Thyroxine (T4): 776.87 g/mol
  • Vitamin D3: 384.64 g/mol
  • Progesterone: 314.46 g/mol
  • Insulin: 5808 g/mol

For example, 10 nM of cortisol is about 3.62 ng/mL, while 10 nM of insulin is about 58.08 ng/mL. The difference exists because insulin has a much higher molecular weight, so the same molar amount represents more mass.

nM to ng/mL Conversion Table

The table below shows how a 10 nM concentration converts to ng/mL for several common molecules using the formula ng/mL = nM × MW ÷ 1000.

Molecule Molecular Weight (g/mol) 10 nM in ng/mL
Cortisol 362.46 3.62 ng/mL
Estradiol 272.38 2.72 ng/mL
Testosterone 288.42 2.88 ng/mL
Thyroxine (T4) 776.87 7.77 ng/mL
Vitamin D3 384.64 3.85 ng/mL
Progesterone 314.46 3.14 ng/mL
Insulin 5808 58.08 ng/mL

Different assays and publications may report the same analyte in either nM or ng/mL. Understanding how molecular weight connects these units makes it possible to compare values accurately across labs, references, and instruments.