Convert U/mL or IU/mL to ng/mL using the substance’s specific activity in U/mg, or convert ng/mL back to U/mL or solve for the specific activity.
U/mL to ng/mL Conversion Formula
The following formula is used to convert U/ml to ng/ml.
Variables:
- U/ml is the concentration of enzyme activity in Units per milliliter.
- ng/ml is the concentration in nanograms per milliliter.
- U/mg is the specific activity
To convert U/mL to ng/mL, divide activity concentration by the matching specific activity (U/mg), then multiply by 1,000,000.
| Enzyme Activity (U/mL) | ng/mL @ 50 U/mg | ng/mL @ 100 U/mg | ng/mL @ 250 U/mg | ng/mL @ 500 U/mg | ng/mL @ 1000 U/mg |
|---|---|---|---|---|---|
| 0.1 | 2000.000 | 1000.000 | 400.000 | 200.000 | 100.000 |
| 0.2 | 4000.000 | 2000.000 | 800.000 | 400.000 | 200.000 |
| 0.5 | 10000.000 | 5000.000 | 2000.000 | 1000.000 | 500.000 |
| 1 | 20000.000 | 10000.000 | 4000.000 | 2000.000 | 1000.000 |
| 2 | 40000.000 | 20000.000 | 8000.000 | 4000.000 | 2000.000 |
| 5 | 100000.000 | 50000.000 | 20000.000 | 10000.000 | 5000.000 |
| 10 | 200000.000 | 100000.000 | 40000.000 | 20000.000 | 10000.000 |
| 15 | 300000.000 | 150000.000 | 60000.000 | 30000.000 | 15000.000 |
| 20 | 400000.000 | 200000.000 | 80000.000 | 40000.000 | 20000.000 |
| 25 | 500000.000 | 250000.000 | 100000.000 | 50000.000 | 25000.000 |
| 30 | 600000.000 | 300000.000 | 120000.000 | 60000.000 | 30000.000 |
| 40 | 800000.000 | 400000.000 | 160000.000 | 80000.000 | 40000.000 |
| 50 | 1000000.000 | 500000.000 | 200000.000 | 100000.000 | 50000.000 |
| 75 | 1500000.000 | 750000.000 | 300000.000 | 150000.000 | 75000.000 |
| 100 | 2000000.000 | 1000000.000 | 400000.000 | 200000.000 | 100000.000 |
| 150 | 3000000.000 | 1500000.000 | 600000.000 | 300000.000 | 150000.000 |
| 200 | 4000000.000 | 2000000.000 | 800000.000 | 400000.000 | 200000.000 |
| 250 | 5000000.000 | 2500000.000 | 1000000.000 | 500000.000 | 250000.000 |
| 500 | 10000000.000 | 5000000.000 | 2000000.000 | 1000000.000 | 500000.000 |
| 1000 | 20000000.000 | 10000000.000 | 4000000.000 | 2000000.000 | 1000000.000 |
| * Rounded to 3 decimals. Formula: ng/mL = (U/mL × 1,000,000) / (U/mg). Columns show ng/mL at illustrative specific activities (U/mg), not substance defaults. Use your actual assay-specific value and its stated protein or material mass basis. Note: 1 µg/mL = 1,000 ng/mL; 1 pg/mL = 0.001 ng/mL. | |||||
What is U/mL to ng/mL Conversion?
U/ml to ng/ml conversion is a process of converting the activity of an enzyme, which is measured in Units per milliliter, to its mass concentration in nanograms per milliliter. This conversion is necessary when working with enzyme assays and biochemical calculations where both activity and mass concentration are relevant.
How to Calculate U/mL to ng/mL?
The following steps outline how to convert U/ml to ng/ml.
- First, determine the enzyme activity in Units per milliliter (U/ml).
- Next, determine specific activity in U/mg for the same material, lot, mass basis and assay. Molecular weight alone cannot convert activity to mass.
- Use ng/mL = (U/mL × 1,000,000) ÷ (U/mg) to calculate mass concentration.
- Finally, choose Mass concentration, enter activity and specific activity with their units, and select Calculate. The other two modes solve activity concentration or specific activity.
Example Problem:
Use the following variables as an example problem to test your knowledge.
Enzyme activity (U/ml) = 2 U/ml
Specific activity = 500 U/mg; mass concentration = (2 × 1,000,000) ÷ 500 = 4,000 ng/mL.
