Enter the enzyme activity in Units per milliliter (U/ml) and the specific activity into the calculator to convert to nanograms per milliliter (ng/ml).
U/Ml to Ng/Ml Conversion Formula
The following formula is used to convert U/ml to ng/ml.
ng / ml = (U / ml) × 1 000 000 ÷ (U / mg)
Variables:
- U/ml is the concentration of enzyme activity in Units per milliliter.
- ng/ml is the concentration in nanograms per milliliter.
- U/mg is the specific activity
To convert U/ml to ng/ml, multiply the enzyme activity (U/ml) by 10^6 times the specific activity.
| Enzyme Activity (U/mL) | ng/mL @ 50 U/mg | ng/mL @ 100 U/mg | ng/mL @ 250 U/mg | ng/mL @ 500 U/mg | ng/mL @ 1000 U/mg |
|---|---|---|---|---|---|
| 0.1 | 2000.000 | 1000.000 | 400.000 | 200.000 | 100.000 |
| 0.2 | 4000.000 | 2000.000 | 800.000 | 400.000 | 200.000 |
| 0.5 | 10000.000 | 5000.000 | 2000.000 | 1000.000 | 500.000 |
| 1 | 20000.000 | 10000.000 | 4000.000 | 2000.000 | 1000.000 |
| 2 | 40000.000 | 20000.000 | 8000.000 | 4000.000 | 2000.000 |
| 5 | 100000.000 | 50000.000 | 20000.000 | 10000.000 | 5000.000 |
| 10 | 200000.000 | 100000.000 | 40000.000 | 20000.000 | 10000.000 |
| 15 | 300000.000 | 150000.000 | 60000.000 | 30000.000 | 15000.000 |
| 20 | 400000.000 | 200000.000 | 80000.000 | 40000.000 | 20000.000 |
| 25 | 500000.000 | 250000.000 | 100000.000 | 50000.000 | 25000.000 |
| 30 | 600000.000 | 300000.000 | 120000.000 | 60000.000 | 30000.000 |
| 40 | 800000.000 | 400000.000 | 160000.000 | 80000.000 | 40000.000 |
| 50 | 1000000.000 | 500000.000 | 200000.000 | 100000.000 | 50000.000 |
| 75 | 1500000.000 | 750000.000 | 300000.000 | 150000.000 | 75000.000 |
| 100 | 2000000.000 | 1000000.000 | 400000.000 | 200000.000 | 100000.000 |
| 150 | 3000000.000 | 1500000.000 | 600000.000 | 300000.000 | 150000.000 |
| 200 | 4000000.000 | 2000000.000 | 800000.000 | 400000.000 | 200000.000 |
| 250 | 5000000.000 | 2500000.000 | 1000000.000 | 500000.000 | 250000.000 |
| 500 | 10000000.000 | 5000000.000 | 2000000.000 | 1000000.000 | 500000.000 |
| 1000 | 20000000.000 | 10000000.000 | 4000000.000 | 2000000.000 | 1000000.000 |
| * Rounded to 3 decimals. Formula: ng/mL = (U/mL × 1,000,000) / (U/mg). Columns show ng/mL at the listed specific activities (U/mg). Note: 1 µg/mL = 1,000 ng/mL; 1 pg/mL = 0.001 ng/mL. | |||||
What is U/Ml to Ng/Ml Conversion?
U/ml to ng/ml conversion is a process of converting the activity of an enzyme, which is measured in Units per milliliter, to its mass concentration in nanograms per milliliter. This conversion is necessary when working with enzyme assays and biochemical calculations where both activity and mass concentration are relevant.
How to Calculate U/Ml to Ng/Ml?
The following steps outline how to convert U/ml to ng/ml.
- First, determine the enzyme activity in Units per milliliter (U/ml).
- Next, determine the molecular weight of the enzyme in Daltons (Da).
- Use the formula ng/ml = U/ml * MW to calculate the concentration in ng/ml.
- Finally, enter the values into the calculator above to verify the result.
Example Problem:
Use the following variables as an example problem to test your knowledge.
Enzyme activity (U/ml) = 2 U/ml
Molecular weight (MW) = 50000 Da