Convert ng/mL and other mass concentrations to M, mM, µM, nM, or pM using molecular weight, or solve for mass concentration or molecular weight.
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ng/ml To Molarity Formula
The following formula is used to calculate molarity from ng/ml.
MW is the molar mass of the exact solute in g/mol. M is mol/L; nM = 1000 × (ng/mL) ÷ MW. A compound-specific shortcut must not be used for a different solute.
| Concentration (ng/mL) | Molarity (nM) |
|---|---|
| 0.1 | 0.333 |
| 0.5 | 1.667 |
| 1 | 3.333 |
| 2 | 6.667 |
| 5 | 16.667 |
| 10 | 33.333 |
| 20 | 66.667 |
| 25 | 83.333 |
| 50 | 166.667 |
| 75 | 250.000 |
| 100 | 333.333 |
| 150 | 500.000 |
| 200 | 666.667 |
| 250 | 833.333 |
| 500 | 1666.667 |
| 750 | 2500.000 |
| 1000 | 3333.333 |
| 2500 | 8333.333 |
| 5000 | 16666.667 |
| 10000 | 33333.333 |
| * Rounded to 3 decimals. Formula: nM = 1000 × (ng/mL) ÷ MW. With MW = 300 g/mol, 1 ng/mL ≈ 3.333 nM. | |
What Does “ng/ml to Molarity” Mean?
ng/ml to molarity is a conversion process used in chemistry to change the concentration of a solution from nanograms per milliliter (ng/ml) to molarity (M). Molarity is a measure of the number of moles of a solute per liter of solution, while ng/ml measures the mass of the solute per volume of solution. The conversion requires knowledge of the molecular weight of the solute, and it is crucial in various scientific fields, including biochemistry and pharmacology.
How to Calculate ng/ml To Molarity?
The following steps use M = C ÷ (MW × 10^6), where C is in ng/mL and MW is in g/mol. Concentration is already per unit volume, so no separate sample volume is required.
- First, determine the concentration in ng/ml (C).
- Next, determine the molecular weight (MW) in g/mol.
- Convert ng/mL to g/L by multiplying by 10^-6.
- Divide that mass concentration in g/L by the molar mass in g/mol.
- Finally, calculate the molarity (M).
- After inserting the variables and calculating the result, check your answer with the calculator above.
Example Problem:
Use the following variables as an example problem to test your knowledge.
concentration in ng/ml (C) = 50
molecular weight (MW) in g/mol = 100
M = 50 ÷ (100 × 10^6) = 5 × 10^-7 mol/L = 0.5 µM = 500 nM. No volume input is needed.
