Enter the A280 and the A260 into the Calculator. The calculator will evaluate the Protein from Layne Equation.

## Protein from Layne Equation Formula

P = 1.55*A280 -.76*A260

Variables:

- P is the Protein concentration from Layne Equation ((mg/mL))
- A280 is the A280
- A260 is the A260

To calculate Protein concentration from Layne Equation,

## How to Calculate Protein from Layne Equation?

The following steps outline how to calculate the Protein from Layne Equation.

- First, determine the A280.
- Next, determine the A260.
- Next, gather the formula from above = P = 1.55*A280 -.76*A260.
- Finally, calculate the Protein from Layne Equation.
- After inserting the variables and calculating the result, check your answer with the calculator above.

**Example Problem : **

Use the following variables as an example problem to test your knowledge.

A280 = 7

A260 = 6