Enter the A280 and the A260 into the Calculator. The calculator will evaluate the Protein from Layne Equation. 

Protein from Layne Equation Formula

P = 1.55*A280 -.76*A260


  • P is the Protein concentration from Layne Equation ((mg/mL))
  • A280 is the A280
  • A260 is the A260

To calculate Protein concentration from Layne Equation,

How to Calculate Protein from Layne Equation?

The following steps outline how to calculate the Protein from Layne Equation.

  1. First, determine the A280. 
  2. Next, determine the A260. 
  3. Next, gather the formula from above = P = 1.55*A280 -.76*A260.
  4. Finally, calculate the Protein from Layne Equation.
  5. After inserting the variables and calculating the result, check your answer with the calculator above.

Example Problem : 

Use the following variables as an example problem to test your knowledge.

A280 = 7

A260 = 6