Enter the amount of protein and the product of the molecular weight of the protein and the volume of the solution into the calculator to determine the protein molarity.

Protein Molarity Formula

The following formula is used to calculate the protein molarity.

M = P / (MW * V)

Variables:

  • M is the molarity of the protein (mol/L)
  • P is the amount of protein (g)
  • MW is the molecular weight of the protein (g/mol)
  • V is the volume of the solution (L)

To calculate the protein molarity, divide the amount of protein by the product of the molecular weight of the protein and the volume of the solution.

What is a Protein Molarity?

Protein molarity is a measure of the concentration of protein in a solution, expressed in moles per liter (M). It is a crucial concept in biochemistry and molecular biology, as it allows scientists to quantify the amount of protein present in a sample. This can be important in various experimental procedures, such as determining the rate of enzymatic reactions, studying protein-protein interactions, or measuring the binding affinity of a drug to its target protein.

How to Calculate Protein Molarity?

The following steps outline how to calculate the Protein Molarity.


  1. First, determine the amount of protein (P) in grams.
  2. Next, determine the molecular weight of the protein (MW) in grams per mole.
  3. Next, determine the volume of the solution (V) in liters.
  4. Next, gather the formula from above = M = P / (MW * V).
  5. Finally, calculate the Protein Molarity (M).
  6. After inserting the variables and calculating the result, check your answer with the calculator above.

Example Problem : 

Use the following variables as an example problem to test your knowledge.

amount of protein (P) = 10 g

molecular weight of the protein (MW) = 50 g/mol

volume of the solution (V) = 0.5 L