Enter the initial concentration and dilution factor into the calculator to determine the final concentration after a 10-fold dilution. This calculator can also evaluate any of the variables given the others are known.

10 Fold Dilution Formula

The following formula is used to calculate the final concentration after a 10-fold dilution.

C2 = C1 / D

Variables:

  • C2 is the final concentration after dilution
  • C1 is the initial concentration before dilution
  • D is the dilution factor (in this case, 10 for a 10-fold dilution)

To calculate the final concentration after a 10-fold dilution, divide the initial concentration by the dilution factor.

What is a 10 Fold Dilution?

A 10-fold dilution is a process in which a substance is diluted to one-tenth of its original concentration. This is typically done by taking one part of the substance and adding nine parts of a diluent, such as water or another solvent. The result is a solution that is 10 times less concentrated than the original. This process can be repeated multiple times to achieve even lower concentrations.

How to Calculate 10 Fold Dilution?

The following steps outline how to calculate a 10 Fold Dilution.


  1. First, determine the initial concentration before dilution (C1).
  2. Next, determine the dilution factor (D). In this case, the dilution factor is 10 for a 10-fold dilution.
  3. Next, use the formula C2 = C1 / D to calculate the final concentration after dilution (C2).
  4. Finally, calculate the 10 Fold Dilution by inserting the values of C1 and D into the formula and solving for C2.
  5. After calculating the result, check your answer for accuracy.

Example Problem:

Use the following variables as an example problem to test your knowledge:

Initial concentration before dilution (C1) = 50

Dilution factor (D) = 10