Enter the initial concentration, initial volume, and final volume into the calculator to determine the final concentration. This calculator can also evaluate any of the variables given the others are known.
Primer Dilution Formula
The following formula is used to calculate the final concentration of a primer after dilution.
FC = (IC * V1) / V2
Variables:
- FC is the final concentration of the primer after dilution
- IC is the initial concentration of the primer
- V1 is the initial volume of the primer
- V2 is the final volume after dilution
To calculate the final concentration of a primer after dilution, multiply the initial concentration of the primer by the initial volume. Then, divide this result by the final volume after dilution.
What is a Primer Dilution?
A primer dilution is a laboratory procedure used in molecular biology to reduce the concentration of a primer solution. Primers are short strands of DNA or RNA that serve as a starting point for DNA synthesis. They are often supplied in high concentrations, which can be too strong for certain experiments. Therefore, they need to be diluted to a suitable concentration. This is done by adding a specific amount of solvent to the primer solution, typically using distilled water or a buffer solution. The resulting diluted primer solution can then be used in various molecular biology techniques such as polymerase chain reaction (PCR) or DNA sequencing.
How to Calculate Primer Dilution?
The following steps outline how to calculate the Primer Dilution.
- First, determine the initial concentration of the primer (IC).
- Next, determine the initial volume of the primer (V1).
- Next, determine the final volume after dilution (V2).
- Next, gather the formula from above = FC = (IC * V1) / V2.
- Finally, calculate the Final concentration of the primer after dilution (FC).
- After inserting the variables and calculating the result, check your answer with the calculator above.
Example Problem :
Use the following variables as an example problem to test your knowledge.
Initial concentration of the primer (IC) = 0.5
Initial volume of the primer (V1) = 10
Final volume after dilution (V2) = 50
