Enter the initial concentration in ng/ul, initial volume in ul, and final volume in ul into the calculator to determine the final concentration after dilution. This calculator can also evaluate any of the variables given the others are known.

Ng/Ul Dilution Formula

The following formula is used to calculate the final concentration after dilution.

C2 = C1 * (V1 / V2)


  • C2 is the final concentration (ng/ul) C1 is the initial concentration (ng/ul) V1 is the initial volume (ul) V2 is the final volume (ul)

To calculate the final concentration after dilution, multiply the initial concentration by the initial volume. Then divide the result by the final volume.

What is a Ng/Ul Dilution?

A Ng/Ul dilution refers to the concentration of a particular substance in a solution, measured in nanograms (Ng) per microliter (Ul). This is commonly used in molecular biology and biochemistry to quantify the concentration of DNA, RNA, or protein in a sample. The dilution process involves reducing the concentration of the substance in the solution, often to make it suitable for a particular experiment or procedure.

How to Calculate Ng/Ul Dilution?

The following steps outline how to calculate the Ng/Ul Dilution using the formula: C2 = C1 * (V1 / V2).

  1. First, determine the initial concentration (C1) in ng/ul.
  2. Next, determine the initial volume (V1) in ul.
  3. Next, determine the final volume (V2) in ul.
  4. Next, insert the values into the formula: C2 = C1 * (V1 / V2).
  5. Finally, calculate the final concentration (C2) in ng/ul.
  6. After calculating the result, check your answer with the calculator above.

Example Problem:

Use the following variables as an example problem to test your knowledge.

Initial concentration (C1) = 10 ng/ul

Initial volume (V1) = 5 ul

Final volume (V2) = 20 ul