Calculate dilution values from initial and final concentration and volume, solving any missing C1, V1, C2, or V2 in ng/µL, µg/mL, mg/L, µL, mL, or L.
Ng/Ul Dilution Formula
The following formula is used to calculate the final concentration after dilution.
C2 = C1 * (V1 / V2)
Formula source: California State Water Resources Control Board – “Units & Conversion Factors” (2016)
Variables:
- C2 is the final concentration (ng/ul)
- C1 is the initial concentration (ng/ul)
- V1 is the initial volume (ul)
- V2 is the final volume (ul)
To calculate the final concentration after dilution, multiply the initial concentration by the initial volume. Then divide the result by the final volume.
What is a Ng/Ul Dilution?
A Ng/Ul dilution refers to the concentration of a particular substance in a solution, measured in nanograms (Ng) per microliter (Ul). This is commonly used in molecular biology and biochemistry to quantify the concentration of DNA, RNA, or protein in a sample. The dilution process involves reducing the concentration of the substance in the solution, often to make it suitable for a particular experiment or procedure.
How to Calculate Ng/Ul Dilution?
The following steps outline how to calculate the Ng/Ul Dilution using the formula: C2 = C1 * (V1 / V2).
- First, determine the initial concentration (C1) in ng/ul.
- Next, determine the initial volume (V1) in ul.
- Next, determine the final volume (V2) in ul.
- Next, insert the values into the formula: C2 = C1 * (V1 / V2).
- Finally, calculate the final concentration (C2) in ng/ul.
- After calculating the result, check your answer with the calculator above.
Example Problem:
Use the following variables as an example problem to test your knowledge.
Initial concentration (C1) = 10 ng/ul
Initial volume (V1) = 5 ul
Final volume (V2) = 20 ul
