Enter the Total Nucleated Cell count, dilution factor, and volume of blood sampled into the calculator to determine the White Blood Cell count. This calculator can also evaluate any of the variables given the others are known.

Wbc Formula

The following formula is used to calculate the White Blood Cell (WBC) count.

WBC = (TNC * D) / V

Variables:

  • WBC is the White Blood Cell count (cells/µL)
  • TNC is the Total Nucleated Cell count (cells)
  • D is the dilution factor
  • V is the volume of blood sampled (µL)

To calculate the White Blood Cell count, multiply the Total Nucleated Cell count by the dilution factor. Then divide the result by the volume of blood sampled.

What is a WBC?

A WBC, or white blood cell, is a type of cell in the body’s immune system that helps fight off infections and diseases. These cells are produced in the bone marrow and are found throughout the blood and lymphatic system. They are crucial for maintaining good health and protection against illnesses. There are several types of white blood cells, including neutrophils, lymphocytes, monocytes, eosinophils, and basophils, each with a specific role in supporting the immune system.

How to Calculate Wbc?

The following steps outline how to calculate the White Blood Cell count (WBC) using the formula: WBC = (TNC * D) / V.


  1. First, determine the Total Nucleated Cell count (TNC) (cells).
  2. Next, determine the dilution factor (D).
  3. Next, determine the volume of blood sampled (V) (µL).
  4. Next, gather the formula from above = WBC = (TNC * D) / V.
  5. Finally, calculate the White Blood Cell count (WBC).
  6. After inserting the variables and calculating the result, check your answer with the calculator above.

Example Problem:

Use the following variables as an example problem to test your knowledge.

Total Nucleated Cell count (TNC) = 1500 cells

Dilution factor (D) = 2

Volume of blood sampled (V) = 100 µL