Enter the Total Nucleated Cell count, dilution factor, and volume of blood sampled into the calculator to determine the White Blood Cell count. This calculator can also evaluate any of the variables given the others are known.
Important: This calculator is for educational use and manual cell-counting math (counted nucleated cells in a known measured volume with an applied dilution, e.g., a counting chamber/hemocytometer). It is not medical advice and is not a substitute for clinician interpretation or a clinical lab report. Reference ranges and units vary by lab method and patient factors (age, pregnancy, conditions), so use the reference interval on your own lab report and discuss abnormal results or symptoms with a licensed clinician. Seek urgent care for concerning symptoms (for example fever, chills, shortness of breath, chest pain, confusion, or feeling severely unwell). For general background on WBC testing and CBC interpretation, consult reputable patient resources such as MedlinePlus or major hospital laboratory guides.
- All Health and Medical Calculators
- Do2 Calculator
- Average Blood Pressure Calculator
- MAP Calculator (Mean Arterial Blood Pressure)
WBC Formula
The following formula is used to calculate the White Blood Cell (WBC) count.
WBC = (TNC * D) / V
Variables:
- WBC is the White Blood Cell concentration (commonly reported as cells/µL, ×103/µL, or ×109/L)
- TNC is the number of nucleated cells counted in the measured sample volume/chamber (cells)
- D is the dilution factor
- V is the measured volume sampled (µL)
To calculate the White Blood Cell count, multiply the Total Nucleated Cell count by the dilution factor. Then divide the result by the volume of blood sampled.
What is a WBC?
A WBC, or white blood cell, is a type of cell in the body's immune system that helps fight off infections and diseases. These cells are produced in the bone marrow and are found throughout the blood and lymphatic system. They are crucial for maintaining good health and protection against illnesses. There are several types of white blood cells, including neutrophils, lymphocytes, monocytes, eosinophils, and basophils, each with a specific role in supporting the immune system.
How to Calculate WBC?
The following steps outline how to calculate the White Blood Cell count (WBC) using the formula: WBC = (TNC * D) / V.
- First, determine the Total Nucleated Cell count (TNC) (cells).
- Next, determine the dilution factor (D).
- Next, determine the volume of blood sampled (V) (µL).
- Next, gather the formula from above = WBC = (TNC * D) / V.
- Finally, calculate the White Blood Cell count (WBC).
- After inserting the variables and calculating the result, check your answer with the calculator above.
Example Problem:
Use the following variables as an example problem to test your knowledge.
Total Nucleated Cell count (TNC) = 1500 cells
Dilution factor (D) = 2
Volume of blood sampled (V) = 100 µL
