Calculate delta absorbance per minute from total absorbance change and time, or estimate enzyme activity in U/mL and U/L from assay inputs.
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Delta Absorbance Per Minute Formula
The following two example problems outline the steps and information needed to calculate the Delta Absorbance Per Minute.
APM = dA / T
Variables:
- APM is the Delta Absorbance Per Minute (absorbance/min)
- dA is the total change in absorbance
- T is the total time (min)
To calculate the delta absorbance per minute, divide the total change in absorbance by the total time.
How to Calculate Delta Absorbance Per Minute?
The following steps outline how to calculate the Delta Absorbance Per Minute.
- First, determine the total change in absorbance.
- Next, determine the total time (min).
- Next, gather the formula from above = APM = dA / T.
- Finally, calculate the Delta Absorbance Per Minute.
- After inserting the variables and calculating the result, check your answer with the calculator above.
Example Problem :
Use the following variables as an example problem to test your knowledge.
total change in absorbance = 5
total time (min) = 3
APM = dA / T = ?
