Calculate delta absorbance per minute from total absorbance change and time, or estimate enzyme activity in U/mL and U/L from assay inputs.
- All Chemistry Calculators
- Absorbance to Transmittance Calculator
- Concentration from Absorbance Calculator
- LogP (Partition Coefficient) Calculator
- Partition Coefficient Calculator
Delta Absorbance Per Minute Formula
The following formula and example show how to calculate the average absorbance change per minute.
Variables:
- APM is the Delta Absorbance Per Minute (absorbance/min)
- dA is the total change in absorbance
- T is the total time (min)
To calculate the delta absorbance per minute, divide the signed absorbance change by a positive elapsed time in minutes. For enzyme activity, use a blank-corrected linear rate and an extinction coefficient appropriate to the wavelength and assay conditions; the widget assumes 1:1 stoichiometry and reports activity of the sample added to the assay.
How to Calculate Delta Absorbance Per Minute?
The following steps outline how to calculate the Delta Absorbance Per Minute.
- First, determine the total change in absorbance.
- Next, determine the total time (min).
- Next, gather the formula from above = APM = dA / T.
- Finally, calculate the Delta Absorbance Per Minute.
- After inserting the variables and calculating the result, check your answer with the calculator above.
Example Problem :
Use the following variables as an example problem to test your knowledge.
total change in absorbance = 5
total time (min) = 3
APM = dA / T = 5 / 3 โ 1.6667 absorbance/min
