Enter the absorbance, path length, and the extinction coefficient into the calculator to determine the concentration.
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Concentration from Absorbance Formula
The following formula is used to calculate a concentration from absorbance.
C = A / (L * e)
- Where A is the absorbance
- L is the path length
- e is the extinction coefficient
A concentration is defined as the total amount of a substance in a given space.
Concentration from Absorbance Example
How to calculate concentration from absorbance?
- First, determine the absorbance.
Calculate the absorbance.
- Next, determine the path length.
Measure the total path length of absorbance.
- Next, determine the extinction coefficient.
Calculate the extinction coefficient.
- Finally, calculate the concentration.
Calculate the concentration using the equation above.
A concentration is a measure of the total amount of substance contained is a certain area or volume.