Transformation Efficiency Calculator

Calculate the bacterial transformation efficiency. Enter the # of colonies per plate, and the total ng of DNA plated into the transformation efficiency calculator below.

Transformation Efficiency Formula

The following formula can be used to calculate the bacteria transformation efficiency.

x = y/z*100

  • Where x is the transformation efficiency (tansformants/ng of DNA)
  • y is the the # of colonies on plate
  • z is the total ng of DNA plated

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